culture conditions human carcinoma lung epithelial cell line a549  (ATCC)

Journal: Applied Microbiology and Biotechnology

Article Title: Addition of lactoferrin increases efficacy of three Kayviruses and limits the inflammatory response in pulmonary epithelial cells

doi: 10.1007/s00253-025-13695-9

Figure Lengend Snippet: Cell viability ( A ), LDH release ( B ), and caspase-1 activity ( C ) of the A549 cells treated with phages vB_SauM-A, vB_SauM-C, vB_SauM-D, 1.0 mg/mL lactoferrin, 2.0 mg/mL linezolid, and bacteriophages supplemented with 1 mg/mL lactoferrin in comparison to the untreated negative control and positive control. Arithmetic mean of triplicates with error bars representing SD. Statistical analysis was performed using one-way ANOVA, with *** p < 0.001

Article Snippet: Human carcinoma lung epithelial cell line A549 (ATCC, CCL-185) (ATCC, Manassas, VA, USA) were cultured in 75-cm 2 Culture Flask (Corning, New York, NY, USA) in a F-12 K culture medium (ATCC, Manassas, VA, USA) supplemented with 10% fetal bovine serum (FBS; ATCC, Manassas, VA, USA), at 37 °C in a humidified atmosphere of 95% air and 5% CO 2 in the HeraCell 150 incubator (Heraeus, Hanau, Germany) in accordance with the supplier’s guidelines.

Techniques: Activity Assay, Comparison, Negative Control, Positive Control

Journal: Applied Microbiology and Biotechnology

Article Title: Addition of lactoferrin increases efficacy of three Kayviruses and limits the inflammatory response in pulmonary epithelial cells

doi: 10.1007/s00253-025-13695-9

Figure Lengend Snippet: The CFU/mL of MRSA strains 70 ( A ), 110 ( B ), and 203 ( C ) in infected A549 cell line after treatment with phages vB_SauM-A, vB_SauM-C, and vB_SauM-D, lactoferrin, linezolid, or phage + Lf combination after 3 h and 6 h post-treatment. Arithmetic mean of triplicates with error bars representing SD. Statistical analysis was performed using the Kruskal–Wallis test followed by Dunn’s multiple comparison test for values with nonparametric distribution, with *** p < 0.001

Article Snippet: Human carcinoma lung epithelial cell line A549 (ATCC, CCL-185) (ATCC, Manassas, VA, USA) were cultured in 75-cm 2 Culture Flask (Corning, New York, NY, USA) in a F-12 K culture medium (ATCC, Manassas, VA, USA) supplemented with 10% fetal bovine serum (FBS; ATCC, Manassas, VA, USA), at 37 °C in a humidified atmosphere of 95% air and 5% CO 2 in the HeraCell 150 incubator (Heraeus, Hanau, Germany) in accordance with the supplier’s guidelines.

Techniques: Infection, Comparison

Journal: Applied Microbiology and Biotechnology

Article Title: Addition of lactoferrin increases efficacy of three Kayviruses and limits the inflammatory response in pulmonary epithelial cells

doi: 10.1007/s00253-025-13695-9

Figure Lengend Snippet: Cell viability ( A , B , C ) and LDH release ( D , E , F ) from the infected A549 cells treated with phages vB_SauM-A, vB_SauM-C, vB_SauM-D, 1.0 mg/mL lactoferrin, 2.0 mg/mL linezolid, and phage + Lf combination in comparison to the untreated negative control and positive control. Arithmetic mean of triplicates with error bars representing SD. Statistical analysis was performed using one-way ANOVA, with *** p < 0.001, ** p < 0.01, and * p < 0.05

Article Snippet: Human carcinoma lung epithelial cell line A549 (ATCC, CCL-185) (ATCC, Manassas, VA, USA) were cultured in 75-cm 2 Culture Flask (Corning, New York, NY, USA) in a F-12 K culture medium (ATCC, Manassas, VA, USA) supplemented with 10% fetal bovine serum (FBS; ATCC, Manassas, VA, USA), at 37 °C in a humidified atmosphere of 95% air and 5% CO 2 in the HeraCell 150 incubator (Heraeus, Hanau, Germany) in accordance with the supplier’s guidelines.

Techniques: Infection, Comparison, Negative Control, Positive Control

Journal: Applied Microbiology and Biotechnology

Article Title: Addition of lactoferrin increases efficacy of three Kayviruses and limits the inflammatory response in pulmonary epithelial cells

doi: 10.1007/s00253-025-13695-9

Figure Lengend Snippet: Inflammasome activation measured by caspase-1 activity in the infected A549 cells treated with phages vB_SauM-A, vB_SauM-C, vB_SauM-D, 1.0 mg/mL lactoferrin, 2.0 mg/mL linezolid, and phage + Lf combination in comparison to the untreated negative control and positive control. Arithmetic mean of triplicates with error bars representing SD. Statistical analysis was performed using the Kruskal–Wallis test followed by Dunn’s multiple comparison test for values with nonparametric distribution, with *** p < 0.001

Article Snippet: Human carcinoma lung epithelial cell line A549 (ATCC, CCL-185) (ATCC, Manassas, VA, USA) were cultured in 75-cm 2 Culture Flask (Corning, New York, NY, USA) in a F-12 K culture medium (ATCC, Manassas, VA, USA) supplemented with 10% fetal bovine serum (FBS; ATCC, Manassas, VA, USA), at 37 °C in a humidified atmosphere of 95% air and 5% CO 2 in the HeraCell 150 incubator (Heraeus, Hanau, Germany) in accordance with the supplier’s guidelines.

Techniques: Activation Assay, Activity Assay, Infection, Comparison, Negative Control, Positive Control

Journal: bioRxiv

Article Title: Aqueous two-phase bioinks for discrete packing and compartmentalisation of 3D bioprinted cells

doi: 10.1101/2025.06.27.661968

Figure Lengend Snippet: Partitioning of different cell types within the various ATPS scaffold formulations. Brightfield and confocal (merged) images representative of cells labelled with DAPI to show the nucleus (blue) of the different cell types (A549, C2C12, MG63, HBMSCs), and GelMA marked with rhodamine B to show the inner phase (red) of the scaffolds at 0 - 9 - 36 g/L. In all cell types, images were taken both under conditions where GelMA was not chemically cross-linked (-GelMA) and under conditions where GelMA was chemically cross-linked (+GelMA). Scale bars: (a, b, c, d) 100 μm. Mean ± S.D. n=3.

Article Snippet: The A549 human lung carcinoma epithelial-like cell line, C2C12 mouse pre-myoblast cell line and MG63 human osteosarcoma cell line were obtained from ATCC (USA).

Techniques: